Effects of Ceftriaxone on Systemic and Central Expression of Anti- and Pro-inflammatory Cytokines in Alcohol-Preferring (P) Rats Exposed to Ethanol

  1. P.S.S. Rao,
  2. S. Ahmed and
  3. Y. Sari*
  1. Department of Pharmacology, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, Toledo, OH, USA
  1. *Corresponding author: Department of Pharmacology, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, Health Science Campus, 3000 Arlington Avenue, Toledo, OH 43614, USA. E-mail: youssef.sari{at}utoledo.edu
  • Received December 13, 2013.
  • Revision requested January 21, 2014.
  • Revision received February 25, 2014.
  • Accepted March 20, 2014.

Aims: Determine the effect of reduction in ethanol consumption by alcohol-preferring (P) rats, following ceftriaxone treatment, on the cytokines levels in prefrontal cortex (PFC) and plasma. Methods: Following 5 weeks of free access to ethanol (15 and 30%), P rats were treated daily with ceftriaxone or saline vehicle for either 2 or 5 consecutive days. Plasma and PFC were collected from ceftriaxone- and saline vehicle-treated groups, and assayed for the levels of pro- and anti-inflammatory cytokines. Results: A significant increase in the plasma level of anti-inflammatory cytokine IL-10 was observed in the ceftriaxone-treated group when compared with the saline-treated group in both the 2-day and 5-day treatments. Furthermore, ceftriaxone treatment for 2 days induced reduction in TNFα level in both plasma and PFC. Additionally, ceftriaxone treatment for 2 days significantly reduced the IFNγ level in PFC. Conclusion: These findings show the ability of ceftriaxone to reduce alcohol consumption and induce modulation of the anti-inflammatory and pro-inflammatory cytokines levels in P rats.